This research explores the development of web based reference software for characterisation of surface roughness for two-dimensional surface data. The aim of the thesis is to develop a web based reference software which makes the evaluation methods for verification of surface characteristics easier for clients. The algorithms used in this software are based on International ISO standards. Most software used in industrial measuring instruments may give variations in the parameters calculated due to numerical changes in calculation. Such variations can be verified using the proposed reference software. The evaluation of surface roughness is carried out in four major steps: data capture, data align, data filtering and parameter calculation. This work walks through each of these steps explaining how surface profiles are evaluated by pre-processing steps called fitting and filtering. The analysis process is followed by parameter evaluation according to DIN EN ISO 4287 and DIN EN ISO 13565-2 standards to extract important information from the profile to characterise surface roughness.