Transnational approaches and theories have reshaped the interdisciplinary trajectory of American Studies since the turn of the millennium. The further extension of perspectives on the United States and North America to prominently include Atlantic Studies, Hemispheric Studies, and Pacific Studies has complicated long-standing notions of ‘American Studies’ and problematized concepts such as nation, identity, and American exceptionalism. The collection gathers thirty original contributions to transnational American Studies from the fields of cultural studies, literature, history, politics, and media studies. Individual essays reassess the global role of the U.S. and its perceptions from within and without, discuss how transnational and comparative explorations emphasize multidirectional processes of cultural exchange and transfer, and show how paradigms of migration and cultural mobility have taken definitions and practices of American Studies beyond traditional geographical and disciplinary limits.